Journal: Channels (Austin, Tex.)

Article Title: Immunomagnetic separation is a suitable method for electrophysiology and ion channel pharmacology studies on T cells.

doi: 10.1080/19336950.2020.1859753

Figure Lengend Snippet: Figure 2. Comparison of viability and purity after the different separation techniques. Viability was determined with 7-AAD staining while purity was expressed as a percentage of CD4+ cells in the living cell population (see Methods). Measurements were made via flow cytometry, subsequently after the separation (values are plotted as mean±SD for the number of independent experiments indicated in the bar). No significant differences were found among the groups for either viability or purity (one-way analysis of variance, p > 0.05). REAlease®: measured after the complete removal of bead-antibody and REAlease® complex, in label-free configuration (see Figure 1), Negative selection: immunomagnetic negative selection, Sorted: positive selection by flow cytometry.

Article Snippet: Immunomagnetic negative selection-based separation (CD4+ T Cell Isolation Kit Cat. No.: 130–096-533 Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany) was prepared to isolate cells without labeling of the CD4 molecule, and these cells served as the “Control” configuration (Figure 1).

Techniques: Comparison, Staining, Flow Cytometry, Selection

Journal: Scientific Reports

Article Title: Dermatophagoides pteronyssinus immunotherapy changes the T-regulatory cell activity

doi: 10.1038/s41598-017-12261-2

Figure Lengend Snippet: Cytokines produced by Teff cells after DP stimulation in absence or presence of Treg cells. Box plots represent comparative analysis of percentages of Teff cells producing IL-10 (CD3 + CD4 + IL-10 + ) ( A ), IL-4 (CD3 + CD4 + IL-4 + ) ( B ) and IL-9 (CD3 + CD4 + IL-9 + ) ( C ) from treated (N = 16) and IL-10 (CD3 + CD4 + IL-10 + ) ( D ), IL-4 (CD3 + CD4 + IL-4 + ) ( E ) and IL-9 (CD3 + CD4 + IL-9 + ) ( F ) from untreated (N = 8) patients in different combinations of Teff and Treg at 0 or 12 months (0 M and 12 M, respectively). Statistical analysis was carried out by Wilcoxon test; p-values ≤ 0.05 are considered statistically significant.

Article Snippet: Treg and Teff cells were isolated from PBMCs at 0 and 12 month using the immunomagnetic EasySep TM Human CD4 + CD127 low CD25 + Regulatory T Cell Isolation Kit (STEMCELL TM Technologies, Vancouver, Canada).

Techniques: Produced